Electrochemical biosensing in food for pathogenic bacteria based on magnetic nanostructured transducers

A novel strategy for electrochemical biosensing of food pathogenic bacteria using nanostructured electrochemical magneto electrode is reported. In this approach, the bacteria are attached to magnetic beads by immunological reaction with specific antibody against Salmonella. Further amplification of the genetic material with a double-tagging set of primer is then performed and the amplicon captured with streptavidin-magnetic beads. The magnetic beads are then attached to an improved nanostructured magnetic transducer for the electrochemical detection, based on bamboo carbon nanotubes. The features of this new approach are compared with classical cultured methods as well as PCR strategies and other electrochemical detection methods based on conventional nonnanostructured transducers. A limit of detection in milk of 1 cfu/mL was obtained with this method.